Study protocol for a prospective cohort study identifying risk factors for sport injury in adolescent female football players: the Karolinska football Injury Cohort (KIC)

This is a prospective observational cohort study designed in agreement with Strengthening the Reporting of Observational studies in Epidemiology (STROBE) guidelines.33

Study setting and participants

Football clubs with adolescent female academy players aged 12–19 years, participating in Swedish divisions 1–2 for girls in the largest regions, are eligible to participate in the study. Most players will be recruited in Stockholm. The district of Stockholm consists of 140 teams and approximate 2520 female players, 13–19 years old. Clubs which meet the inclusion criteria are contacted and invited to participate and are given oral and written information. Clubs which choose to take part in the study are provided with a more detailed oral and written information in the presence of players, legal guardians and coaches.

A cohort of approximately 400 adolescent academy players will be recruited. An internal pilot study of 63 football players has been conducted to test the infrastructure and the implementation of the study, with satisfactory results (unpublished data).

Baseline measurements

Questionnaires

The baseline questionnaire covers potential risk factors for the aetiology of sport injuries as well as information about players’ general health status. Players are surveyed in various areas, including health: health problems (eg, illness), medication, age at menarche, amenorrhoea; lifestyle: sleep patterns, eating habits, food supplements, tobacco as smoking or Swedish snus (snuff) and alcohol; and socioeconomic factors: guardians’ education. Included football-related factors are: training and match play exposure, playing position, dominant limb, years of experience, other sports participation, injury preventive strategies (eg, the Swedish injury prevention warmup programme Knee Control)34 and type of turf at the home facilities (artificial or natural grass) according to guidelines for football studies.35–37 Psychosocial factors are surveyed using: the modified General Health Questionnaire-12 consisting of 12 items regarding self-reported general psychological health using a four-point Likert scale,38 coping assessed by a 28 item self-report questionnaire that measure effective and ineffective strategies to cope with stressful events using a four-point Likert scale (Brief COPE),39 player’s passion to sport measured in harmonious and obsessive passions using a 14 item questionnaire with a seven-point Likert scale (Passion scale),40 education in sport psychology, regularly seeing a sport psychologist/mental coach and perceived stress (single item question).41 Injury history: injuries occurring within the previous 6 months prior to inclusion are captured using a modified Swedish version of the validated psychometric instrument Oslo Sports Trauma Research Center Overuse Injury Questionnaire (OSTRC-O).42 43 Back and neck pain is covering the frequency, intensity, disability of low back pain (LBP) and upper back pain/neck pain (UBNP) and corresponding longitudinal trajectories the preceding 6 months using modified versions of the Chronic Pain Questionnaire44 and Visual Trajectories Questionnaire-Pain,45 respectively.

In addition, coaches in the included teams are surveyed regarding their education, years of experience, the use of warmup and stretching regime and implementation of injury prevention programmes.

Physical test protocol

The physical test protocol includes several tests that are considered valid, reliable, and field friendly; performed in approximately 60 min/player. The protocol comprises measurements of strength, mobility and control of lower extremity, trunk and neck and also include anthropometric measurements (height, weight and leg length). The protocol is briefly outlined below, however, further details including visual presentations is available in the electronic supplementary file (online supplemental file 1).

Supplemental material

All test procedures are conducted in indoors facilities during weekends. The physical tests are divided into nine test stations with 1–2 test leaders each (online supplemental file 1). Hitherto, 52 clinically experienced test leaders have been involved in data collection. They were trained by MA, VL, NW and the previous test leader in charge of the station to ensure consistent execution and reliability. Information and instructions given to the players regarding the tests are standardised, and test leaders refrain from coaching or encouraging the players in any way during the procedures.

A maximum of nine players are tested per session (ie, one at each station) and are informed to train and compete as usual prior to testing. Players are informed to refrain from certain tests that evoke pain, provoke ongoing injuries or other health-related issues. Prior to performing the physical tests, players complete a standardised 7 min warm-up programme comprising 4 min of jogging, 10×1 body weight squats, 10×1 body weight squat jumps and 10×1 unilateral body weight lunges. Following the warm-up session, players are randomly assigned to a starting test station and subsequently follow a predefined order.

Calf heel raises

Ankle plantarflexion (PF) muscle endurance is investigated using unilateral weight bearing calf heel raises.46 The player is instructed to perform maximum unilateral barefoot heel raises continuously to failure, guided by a metronome to standardise the pace (1 s concentric, 1 s eccentric contraction). The test leader registers the number of accomplished repetitions and discontinues when the player fails to reach the marked target height. The same procedure is then conducted on the opposite foot.

Active PF mobility

Active PF ROM is measured with a universal goniometer in supine position using fibula and fifth metatarsal as reference marks.47 48 The player is instructed to maintain extended knees throughout the movement, and to perform a sequence of six maximal active PF cycles from a neutral dorsiflexion (DF) position, of which the final three trials are registered.

Weight bearing ankle DF mobility

Weight bearing ankle DF ROM is measured in a lunge position with the player’s foot placed on a metric ruler 10 cm away from a wall.46 49 The player is instructed to lunge forward, until contact with the wall is achieved without allowing the heel to lift off the ground. Three warm-up trials are performed from the 10 cm mark to familiarise the player with the test. Thereafter, the test leader measures the following three trials. From the 10 cm reference mark, the player progresses 1 cm away at a time from the wall until unable to perform a successful repetition. If unable to perform a successful repetition at the 10 cm reference mark, she is asked to progress 1 cm forward until able to complete a successful repetition. The maximal DF ROM is measured with a digital inclinometer (Clinometer, Plaincode, Stephanskirchen, Germany) and distance from the wall to the greater toe is measured in centim.

Trunk mobility

Trunk rotation mobility is measured in a modified seated rotation test, and in a lunge position on a gym mat graded with 5° increments.50–52 The player is instructed to maximally rotate alternating between right and left, in a cross-legged position and subsequently in a lunge position on the dominant, and non-dominant limb while the test leader measures the rotational degrees in the end range. Three repetitions are performed in each direction during the three separate positions, and the mean value for each position is later used for analysis.

Trunk strength

Isometric trunk rotational strength is measured in a modified standing wood chopper test using a force gauge to evaluate force output (RS Pro Digital Force Gauge, RS Components, Corby, UK).53–55 In this modified test, the player holds a handle attached to the force gauge in shoulder height in a standing position. The player is instructed to generate force through her trunk and rotate for 5 s while maintaining straight arms. Three consecutive repetitions are conducted in each direction and the maximal force output is later used for analysis.

Deep neck flexor endurance

Deep neck flexor muscle endurance is assessed through a modified version of the cranio-cervical flexion test with a pressure sensor (Stabilizer Pressure Bio-Feedback, Chattanooga Group, Hixon, Tennessee, USA).51 56 57 The test consists of a pretest and an endurance test. In the pretest the player is positioned in a supine position on an examination table and are instructed to slightly push the neck against the pressure sensor to increase the pressure and then maintain the pressure for 3×3 s, with a 3 s rest in between each contraction, at a specific target pressure (TP), starting at 20 mm Hg. If the player can perform this task, she is instructed to increase the pressure to 24 mm Hg and keep the pressure for another 3×3 s. This is repeated with a 2 mm Hg increase until the player reaches 30 mm Hg. If the player can perform the pretest the endurance test is subsequently performed. During the endurance test, the same setup and procedure as in the pretest is carried out. However, the player is instructed to hold each contraction at the TP for 3×10 s with a 10 s rest in between contractions. The highest completed TP with a full set of 3×10 s contractions is later used for analysis.

Hip and knee strength

Isometric hip flexion, extension, adduction and abduction strength as well as eccentric hip abduction and adduction strength are measured with a hand-held dynamometer (HHD) (MicroFet2, Hoggan Health Industries inc. West Jordan, Utah, USA).58 59 Furthermore, isometric knee extension strength is measured with an HHD and the player in a seated position with the knee joint in 90° of flexion. Prior to executing the strength tests, two submaximal isometric contractions in each direction are performed to familiarise the player with the procedures. Three isometric contractions with gradually increasing power output for 5 s, and three maximally eccentric contractions for 3 s are performed in the isometric and eccentric tests, respectively, with a 10 s rest in between contractions. The maximal power output for each position is later used for analysis.

Hip mobility

Measures of passive hip ROM in flexion and abduction in prone position and extension, internal and external rotation in supine position is obtained using a universal goniometer.60 61 Three consecutive measurements for each position are performed for both the dominant and the non-dominant leg, and the mean value for each position is later used for analysis.

Functional performance tests

To assess the player’s unilateral jump performance, the One-leg Long Box Jump Test (OLLBJ) and square hop test are performed.62 63 A 40×40 cm square is marked on the foundation and later used as a reference mark in both tests.

In the OLLBJ, the starting position are calculated by dividing the player’s height (cm) with 1.6 (height/1.6). Thereafter, the player is instructed to stand on one leg on the starting position and then jump on one leg directed inside the boundaries of the square and maintain balance after landing. Three warm-up trials and five consecutive test trials are performed on each leg. The total number of approved trials are registered by the test leader.

During the square hop test, previously described in detail,62 63 the player is instructed to jump on one leg in and out of the square as many times as possible for 15 s in a clockwise direction, timed with a stopwatch while the test leader registers the number of approved jumps. The player performs two warm up trials on each foot prior to executing the test.

Ankle and knee stability

To assess stability of player’s talocrural joints, a modified anterior drawer test is employed.64 65 Furthermore, a modified version of Fairbank’s apprehension test is used to evaluate the player’s stability in the patellofemoral joint.66 The tests are conducted on both the dominant and non-dominant foot and knee and are considered positive if the player experience any pain or discomfort during the examination, and/or an involuntary contraction of the quadriceps musculature occur during the Fairbank’s apprehension test.

Isometric back extensor endurance

Isometric back extensor endurance is assessed by the modified Sorensen test.67–69 In this previously described modified test,67 68 the player’s lower body is supported by an examination table in prone position with three straps and the anterior–superior iliac spine is aligned with the edge of the table. The player is instructed to keep her arms folded across the chest throughout the procedure and isometric maintaining the upper body in a horizontal position until failure. The test leader registers the time elapsed until failure. A digital inclinometer (Clinometer, Plaincode, Stephanskirchen, Germany) is placed on a metric ruler at the level of the 5th vertebra in the thoracic spine to monitor sagittal plane movement. Prior to the assessment, the player completes a shorter warmup trial to orient the desired sagittal plane target angle.

Follow-up measurement and outcome

Follow-up measurements are collected prospectively during 1 year from the baseline. In the weekly online questionnaire, the players are asked to answer questions regarding new and ongoing injuries, LBP and UBNP intensity, social support, perceived stress, recovery, and to be able to consider workload, number of training and match play hours/week.70 To assess whether players sustain football related injuries throughout the follow-up period, the Swedish version of OSTRC-O is employed and included in the weekly online questionnaire.42 43 71 Two study specific adaptions were made to the OSTRC-O. First, a question regarding absence/reduced participation in training/match due to reasons not related to injuries was added. Second, the option to specify injuries in different anatomical localisations in the lower and upper extremity, back, neck, head and abdomen was included.

Football related injuries reported with the OSTRC-O in the weekly online questionnaire leading to moderate or severe reductions in participationand/ or sports performance or complete inability to participate in sport are classified as a substantial injury in this study.42 Players reporting new substantial injuries are contacted via telephone by a clinically experienced research assistant to answer a standardised interview with questions concerning the injury such as: injury mechanism, localisation, type, time-loss, re-injury, diagnosis and medical care. Injuries are divided into acute and gradual onset. An acute injury is defined as a result from a specific, identifiable event, whereas injuries with gradual onset are defined as an injury without a single, identifiable event responsible for the injury.35 Players receive an automated link to the online questionnaire sent by email each Sunday, with a reminder email the next day to players not responding. Furthermore, if no response is received, a text message reminder with the link is sent on Tuesdays. Finally, every other week representatives of the study visit participating football clubs to collect unanswered surveys for the previous 2-week period.

After 52 weeks of participation, a questionnaire with equivalent content as the baseline questionnaire (excluding OSTRC-O with 2 months and 3–6 months recall) are distributed to the players to evaluate possible changes from the baseline characteristics. The first 106 included players also underwent a secondary physical test protocol after 52 weeks of follow-up. In the 1-year follow-up questionnaire, different aspects of UBP and LBP, respectively, in the preceding 6 months are measured. ‘Have you had UBP/Have you had LBP’ (Yes/No)? If yes, has the pain hindered your daily activities (No, Yes to some extent or Yes to a high degree)? If Yes, the ‘VTQ-P’ is used to capture the longitudinal state of a player’s pain experience of UBP and LBP and are retrospectively reported for the preceding 6-month period.45 See table 1 for an overview of the measurements during the different phases of the study.

Table 1

Summary of the included measurements during the different phases of the study

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